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전체arXiv CS.AI6,534arXiv Math6,523arXiv Physics2,314arXiv Stat1,122PLOS ONE423arXiv Q-Bio318arXiv Econ316PLOS Global Public Health61PLOS Biology24PLOS Medicine14
PLOS ONE

Reinforcement learning for policymaking in epidemic control: A scoping review

by Oleksandr Bolshov, Dmytro Chumachenko Background Managing an epidemic demands policies that respond at the pace of the outbreak. Conventional rule‑based interventions struggle to keep up, prompting interest in reinforcement learning (RL) for designing non‑pharmaceutical interventions (NPIs). However, current evidence is fragmented across diverse models and reporting styles. Objectives To systematically map how RL is applied for epidemic NPI design, describe modeling choices, algorithm architectures, evaluation practices, and identify trends and research gaps. Methods Peer-reviewed studies (2014–2025, English) that applied deep RL to select NPIs were retrieved from IEEE Xplore, ACM Digital Library, ScienceDirect, and Scopus, searched on December 23, 2025. Reference list scanning supplemented database results. Predefined data items (bibliographic details, epidemic and RL model characteristics, experiments, validation methods, outcomes) were charted and summarized descriptively. Results Of 512 retrieved records, 10 met the inclusion criteria, and three additional studies were identified via reference-list scanning, yielding 13. Five employed value‑based methods, four policy‑gradient, and four hybrid; one study additionally incorporated model-based planning. Six simulations relied on compartmental models, six on agent‑based models, and one on a hybrid model. Action spaces were predominantly discrete restriction levels. Five studies incorporated sequence-modeling techniques to include temporal context into a state space. Eleven studies designed reward functions as a trade-off between pandemic severity and socio-economic cost. According to the reviewed studies, RL policies across various settings outperform heuristic, rule-based, and historical baselines in reducing infections, deaths, or lockdown duration while limiting economic loss. Conclusions RL shows promise for adaptive epidemic control. Comparison is hampered by simplified economic costs, inconsistent calibration rigor, varied evaluation metrics, and limited uncertainty or policy robustness analysis. Future work should establish common benchmark environments and reporting standards, incorporate empirically grounded economic and behavioral models, adopt uncertainty-aware and probabilistic RL, develop more sophisticated control spaces, investigate more advanced algorithms, and validate learned policies prospectively to enable real-world deployment.

PLOS ONE

Retinal microvascular alterations in children with amblyopia

by Christiane Al-Haddad, Hanadi Ibrahim, Nagib Salameh, Zahi Wehbi, Dalia El Hadi, Youssef Zougheib, Ziad Bashshur Purpose To investigate and compare retinal vascular changes, including superficial macular vessel density, foveal avascular zone, and the macular perfusion densities, in amblyopic, fellow, and control eyes using optical coherence tomography angiography. Methods In this prospective cross-sectional study, we recruited 78 participants, including 36 with amblyopia and 42 controls, to investigate retinal parameters using optical coherence tomography angiography (OCTA) by comparing amblyopic eyes to both controls and fellow eyes. Results A total of 78 patients were enrolled, including 36 with amblyopia (mean age: 9.8 ± 4.6 years) and 42 controls (mean age: 10.0 ± 2.9 years). Among the amblyopic cohort, 18 (50%) had strabismic and 18 (50%) had anisometropic amblyopia. Baseline characteristics, including age, sex distribution, and spherical equivalent, were comparable across groups. Macular OCTA demonstrated higher outer and full macular vessel densities, as well as elevated outer and full regions perfusion densities, in amblyopic eyes relative to control. Optic disc OCTA parameters were comparable between amblyopic eyes and controls. Comparison between amblyopic and fellow eyes showed a significantly smaller macular foveal avascular zone area, with no other significant interocular differences in macular or optic disc OCTA findings. Fellow eyes and control eyes differed only in optic disc perfusion density in the outer region, which was decreased in fellow eyes. Conclusion Amblyopic eyes showed increased outer and full macular vessel and perfusion densities compared with control eyes, and a smaller foveal avascular zone area relative to fellow eyes. No significant microvascular differences were found in the optic disc OCTA among groups. These exploratory findings raise the possibility that amblyopia may be associated with subtle macular microvascular alterations, however, confirmatory studies with larger cohorts are needed.

PLOS ONE

Perspective of Turkish society toward autistic individuals: Personal experiences, knowledge, and interaction comfort

by Gamze Alak Despite increasing awareness of autism spectrum disorder (ASD) and autistic individuals, factors shaping social interactions involving autistic individuals in the general population remain relatively underexplored. This study examined the interrelationships among personal experience, knowledge, and interaction comfort toward autistic individuals among Turkish adults and explored demographic differences. A total of 507 participants (aged 18+) were recruited using snowball sampling, and data were collected via an online survey. Data were analyzed using descriptive, comparative, and correlational analyses, followed by regression-based mediation and moderated mediation models (PROCESS macro). Participants primarily reported indirect experiences with autistic individuals, and both knowledge levels and interaction comfort were relatively high, with significant differences across several demographic characteristics. Interaction comfort was lower in social than in professional settings and varied according to levels of support needs. Knowledge mediated the relationship between personal experience and interaction comfort. The indirect effect was significant only at higher levels of interaction quality. These findings highlight the role of knowledge and interaction quality in shaping interaction comfort and suggest the importance of interventions that promote meaningful and informed interactions. Implications for future research and practice are discussed.

PLOS ONE

Expression of melanoma differentiation–associated gene 5 in the epidermis and cutaneous deposition of complement C3 and immunoglobulins in patients with dermatomyositis

by Yoshiaki Zaizen, Takuma Koga, Masahiro Tsutsumi, Shinjiro Kaieda, Jun Akiba, Takekuni Nakama, Tomoaki Hoshino Objectives Dermatomyositis (DM) is an autoimmune disease characterized by interface dermatitis, but the immunopathological features underlying cutaneous inflammation remain incompletely understood. The aim of this study was to characterize the cutaneous deposition of complement and immunoglobulins, as well as to clarify the localization of melanoma differentiation-associated gene 5 (MDA5) in DM skin. Methods Skin biopsy specimens from 22 patients with DM and 13 control specimens obtained from cancer-free skin of patients with dermatofibrosarcoma protuberans were examined. Immunohistochemical staining for complement C3c, immunoglobulins (IgG, IgM, and IgA), and MDA5 was semi-quantitatively evaluated, focusing on the superficial dermis near the dermo-epidermal junction. Results Significantly greater deposition of C3c, IgM, and IgA was exhibited by DM skin compared with control skin (all p ≤ 0.001), predominantly localised to the superficial dermis at sites of interface dermatitis. In contrast, IgG showed comparable deposition in both DM and control skin. MDA5 was strongly expressed in the stratum spinosum and basal layer of the epidermis in both DM and control skin. Enhanced MDA5 expression was notably observed in dermal inflammatory cells and capillaries in DM skin, but minimal expression was observed in the dermis of control skin. Conclusions DM skin is characterized by the deposition of immunoglobulins and complement C3c at sites of interface dermatitis, findings that are consistent with immune complex-mediated injury. MDA5 is widely expressed in both DM and control skin epidermis and can be detected in the infiltrating inflammatory cells of DM.

PLOS ONE

Correction: Salidroside protects against high-altitude hypoxia-induced kidney injury via regulation of renal dopamine D1-like receptors

by Cheng Huan, Gan Zhilin, Xiao Dan, Wang Yue, Li Xianglian, Mo Liwen, Cheng Yue

PLOS ONE

Correction: The additive effect of IgE-mediated and pseudoallergic hypersensitivity in RBL-2H3 cells and guinea pigs

by Yu Zhang, Qilong Xu, Chengbo Zheng, Cunyu Li, Yunfeng Zheng, Guoping Peng

PLOS ONE

Association between hypnotic medication use and in-hospital falls among older adults: A multicenter landmark analysis

by Takuya Nishino, Yoshiaki Kubota, Yasuo Miyagi, Nari Tanabe, Fumiko Yamaguchi, Hiroki Ito, Shizuka Soh, Ayako Yano, Masako Mizuno, Chol Kim, Yosuke Ishii, Yukihiro Kondo, Kuniya Asai Background Hypnotics are frequently prescribed to hospitalized older adults, but comparative evidence on fall risk across hypnotic classes remains inconsistent, partly due to confounding by indication and inadequate consideration of time at risk during hospitalization. Methods We conducted a retrospective multicenter landmark analysis using administrative claims and electronic medical records from two acute-care hospitals in Japan (2018–2024). A day-7 landmark was defined, including patients aged ≥65 years who remained hospitalized and fall-free through hospital day 7. Sustained hypnotic exposure during hospital days 4–7 (≥2 days) was categorized as no sustained use, benzodiazepines/Z-drugs (BZ/Zs) alone, orexin receptor antagonists or ramelteon (ORA/Ram) alone, or combination therapy. The primary outcome was time to first in-hospital fall during hospital days 8–37. Cox proportional hazards models with multiple imputation were used. Competing-risk analyses and propensity score–matched comparisons between single-class users were conducted as sensitivity analyses. Results Among 61,663 patients, 3,884 (6.3%) experienced an in-hospital fall after the landmark. Compared with no sustained hypnotic use, adjusted hazard ratios (HRs) for falls were 1.42 (95% CI, 1.24–1.64) for BZ/Zs, 1.46 (95% CI, 1.25–1.70) for ORA/Ram, and 1.42 (95% CI, 1.05–1.91) for combination therapy. Results were consistent in competing-risk analyses. In propensity score–matched analyses restricted to single-class users, fall risk did not differ significantly between BZ/Zs and ORA/Ram (adjusted HR, 0.98; 95% CI, 0.75–1.28). Conclusions Sustained hypnotic use during hospitalization was associated with a higher incidence of in-hospital falls among older adults. After adjustment for measured clinical factors and treatment selection, no significant difference in fall risk was observed between BZ/Zs and ORA/Ram.

PLOS ONE

Spurious effects in random-intercept cross-lagged panel models: Results from simulations and reanalyses of data on self-esteem and problematic eating behaviors used by Beckers et al. (2023)

by Kimmo Sorjonen, Gustav Nilsonne, Ata Ghaderi, Bo Melin The random-intercept cross-lagged panel model (RI-CLPM) is an extension of the traditional cross-lagged panel model. The RI-CPLM specifically addresses prospective effects within individuals. In the present simulations, we found that the RI-CLPM is susceptible to spurious findings when observed scores on the two variables are affected by common auto-correlated state factors. In reanalyses of empirical data, we found contradictory decreasing, increasing, and null prospective effects between within-individual levels of self-esteem and problematic eating behaviors among Dutch teenagers (N = 1856). These contradictory findings indicated that previously reported prospective effects may have been statistical artifacts. Caution is advised when using the RI-CLPM, as it may produce misleading results. Researchers are recommended to validate findings using alternative analyses, e.g., by examining person-mean centered scores.

PLOS ONE

Correction: Resilience of the gelatinous zooplankton species <i>Oikopleura dioica</i> to ocean alkalinity enhancement

by The PLOS One Staff

PLOS ONE

Advanced glycation end product accumulation was associated with renal function impairment in males in large health examination population

by Ryo Asaoka, Shigeki Muto, Akira Obana The accumulation of advanced glycation end products (AGEs) is a risk factor for renal dysfunction. However, no investigation has been conducted on the association between AGEs and renal function in health screening participants. Therefore, this study aimed to examine the association between AGEs and kidney function in health screening participants who underwent health screening examination. Overall, 1,651 health screening examinees without a history of renal dysfunction diagnosis were recruited and AGE accumulation was measured by skin autofluorescence (SAF). The association between estimated glomerular filtration rate (eGFR) and AGEs was subsequently investigated in all examinees. The mean age was 56.6 ± 9.5 years; 812 (49.1%) were males, and the mean eGFR was 73.3 ± 13.4 mL/min. Multiple regression analysis identified that AGEs were significantly negatively associated with eGFR. This finding was also observed in examinees with normal eGFR (≧ 90 mL/min/1.73 m2, G1 stage according to the Japanese Society of Nephrology: N = 207). In conclusion, skin AGEs were significantly negatively associated with eGFR. To clarify whether AGEs contribute to renal dysfunction progression, additional research is required.

PLOS ONE

Topological data analysis for predicting disease outbreaks in humanitarian settings: A machine learning approach

by Job Agba Opue, Uchechukwu Emena Okorie, Victor Ede Itita Background Humanitarian settings are highly vulnerable to infectious disease outbreaks because displacement, crowding, disruption of health services, insecurity, and inadequate water and sanitation often interact in ways that are difficult to capture with conventional prediction models. There is a need for forecasting approaches that can integrate heterogeneous data sources and better represent complex system structure. Methods We developed and evaluated a machine-learning framework incorporating topological data analysis to predict cholera and measles surge events (binary indicators per LGA-week) across 97 Local Government Areas in Nigeria between 2018 and 2023. These 97 LGAs represent a selected high-burden subset (12.5%) of Nigeria’s 774 LGAs with sufficient surveillance data. Weekly district-level predictors included climate, conflict, displacement, health-system, and socioeconomic variables. Persistent homology was used to derive topological summaries from multivariate risk profiles, and these were combined with selected raw predictors in gradient-boosting models. Outcomes were defined using surveillance-based outbreak thresholds with a 4-week prediction horizon. Model performance was assessed using temporally ordered hold-out validation, with evaluation of discrimination, calibration, and incremental value over baseline models. Results The topological models achieved ROC-AUC of 0.78 (95% CI: 0.74–0.82) for cholera and 0.81 (95% CI: 0.77–0.85) for measles, representing modest improvements of 0.08–0.12 over models using only conventional predictors. At the optimal decision threshold determined using Youden’s index on validation data, sensitivity was 0.72 (range across folds: 0.68–0.76) and specificity was 0.82 (range: 0.79–0.85) for cholera, with false alert rates varying from 2.8–3.6 per LGA per year across temporal folds. Topological features contributed 35% of predictive importance. Calibration slopes were 0.94 (cholera) and 0.97 (measles). Conclusions Topological feature representations provide a modest but meaningful complementary approach for outbreak prediction in complex humanitarian environments. Their value appears to lie in summarizing higher-order structure across multiple interacting risk domains, rather than replacing established epidemiologic indicators. However, routine deployment requires prospective validation and context-specific threshold tuning. Further external validation, operational threshold analysis, and prospective testing are needed before routine deployment in public-health early warning systems.

PLOS ONE

J-shaped relationship between stress hyperglycemia ratio and delirium risk in critically ill patients: A population-based study

by Yingyang Li, Mengyuan Qiao, Hui Yang, Lu Chen, Haiyan Wang Background The incidence of delirium in critically ill patients is strongly correlated with poor prognosis. The stress hyperglycemic ratio has emerged as a novel marker for assessing the response to acute hyperglycemia. Glycemic fluctuations during periods of stress play a crucial role in precipitating or directly causing delirium. However, the association between SHR and delirium in hospitalized ICU patients remains uncertain. Objective This study aimed to investigate the potential relationship between SHR and delirium in ICU patie nts and to examine possible subgroup differences in this association. Methods A total of 2,093 Intensive care unit (ICU) patients were included in this retrospective cohort study. The relationship between SHR and delirium was explored using multifactorial logistic regression, subgroup analyses, smoothed curve fitting, and threshold effect analysis models. Results Among the 2,093 participants, 59.05% were male and 40.95% were female, with a mean age of 64.19 ± 16.31 years. We identified a non-linear positive correlation between SHR and delirium, with an inflection point at 0.68, and the odds ratio (95% CI) after the inflection point was 1.88 (1.35, 2.62), P < 0.001. This interaction was statistically significant concerning the APACHE II scores and C-reactive protein levels at admission. Conclusion We found a nonlinear positive association between SHR and delirium. Our study highlights that managing SHR levels in critically ill patients may help to prevent or mitigate the development of delirium, emphasizing the potential value of SHR as an early intervention and precision treatment for delirium.

PLOS ONE

RNA metagenomic profiling of mosquito viromes associated with Vector-Borne diseases in Quebec, Canada

by Ines Levade, Benjamin Delisle, Éric Fournier, Christian Therrien Mosquitoes harbor diverse viral communities, including both medically important arboviruses and insect-specific viruses, yet the viromes of mosquito populations in northern temperate regions remains poorly characterized. In this study, we used metagenomic sequencing to analyse pools of archived mosquito samples from Québec, Canada representing multiple species previously identified as arbovirus carriers. Our analyses identified 60 viral species, including three arboviruses, several insect-specific viruses, and multiple dual-host non-pathogenic viruses, revealing the rich viral diversity present in these mosquito populations. Phylogenetic analysis of complete viral genomes demonstrated genetic relationships with viruses reported from diverse geographic regions. We describe, a newly proposed bipartite Culex tombus-like virus and report the complete resolution of thirty-five viral genomic sequences. These results highlight the utility of metagenomic approaches for comprehensive characterization of the mosquito virome and underscore their potential to enhance surveillance of emerging arboviruses, including West Nile virus, in Québec and similar northern ecosystems.

PLOS ONE

Focusing on legal cases: Automatic classification of legal documents with sentence embeddings and deep learning models

by Fawaz Khaled Alarfaj The justice system is indispensable to any society as it enforces the rule of law, safeguards fundamental rights, and ensures the equitable resolution of disputes through structured legal frameworks. Artificial Intelligence (AI) has significantly advanced the legal and justice system by automating time-intensive tasks such as document review and contract analysis, thereby enhancing efficiency and reducing human error. Additionally, AI-powered predictive analytics and decision support systems have improved access to justice by providing data-driven insights, enabling faster case resolution, and ensuring more consistent application of the law. Legal document classification using AI techniques is imperative as it enables efficient organization, retrieval, and analysis of vast volumes of legal texts, enhancing accuracy, reducing manual effort, and facilitating faster decision-making in legal processes. In this research study, the main aim is to classify legal text documents using Machine Learning (ML) and state-of-the-art Deep Learning (DL) algorithms. Using a real-world dataset that consists of thousands of legal documents having complex language related to legal cases poses a challenging natural language understanding task by applying various textual features, deep features, and advanced sentence embeddings. The results reveal that the ensemble learning model of Extremely Randomized Trees shows better results with 89% accuracy, as it aggregates the results of multiple decorrelated decision trees to enhance predictive accuracy and control over-fitting. However, the best results of 96% are achieved with sentence embeddings. Sentence embeddings with Long Short-Term Memory (LSTM) networks are highly effective in Natural Language Processing (NLP) due to their ability to capture complex semantic and syntactic information within text.

PLOS ONE

Single-cell profiling of kinase substrate phosphorylation by single-molecule imaging

by Takuya Hidaka, Ryotaro Motoya, Gao Jintian, Sooyeon Kim, Yuichi Taniguchi Protein phosphorylation regulates diverse cellular processes, yet its analysis at the single-cell level remains challenging due to the low abundance of phosphoproteins. Here, we present a highly sensitive system for profiling phosphorylation of kinase substrates in individual cells. The method integrates fluorescence labeling of single-cell proteomes, immunoprecipitation using antibodies recognizing phosphorylation within specific amino acid motifs, miniaturized SDS-PAGE, and single-molecule detection using a custom-built light-sheet fluorescence microscope. We applied this approach to analyze substrates of casein kinase 2 (CK2) in HeLa cells treated with the phosphatase inhibitor calyculin A. Bulk and pseudo-single-cell analyses confirmed treatment-induced accumulation of phosphorylated CK2 substrates and demonstrated quantitative performance over biologically relevant input ranges. Importantly, true single-cell measurements revealed heterogeneous phosphorylation patterns across molecular weight regions, highlighting cell-to-cell variability in CK2 signaling that is obscured in bulk analyses. This platform enables profiling of the phosphorylation states of a wide range of kinase substrates in individual cells and provides a foundation for dissecting heterogeneous signaling dynamics.

PLOS ONE

Navigating the digital era: The impact of digitalization and work-life harmony on well-being among solo self-employed individuals

by Hyeon Jo, Hyunchul Ahn In an era where technological advancements and work-life integration significantly shape the professional landscape, understanding their impact on individual job satisfaction and well-being is crucial, particularly for self-employed business owners. This study explores the effects of digitalization, autonomy, work-life balance, work engagement, and burnout on the job satisfaction and well-being of the self-employed. Using Partial Least Squares Structural Equation Modeling (PLS-SEM) on a sample of 12,703 respondents from the Sixth Korean Working Conditions Survey (2020), this research offers comprehensive insights into the unique challenges faced by this demographic. The findings indicate that digitalization and automation significantly increase technology anxiety. In contrast, leadership autonomy and responsibility enhance job satisfaction but adversely impact well-being. Work-life interference negatively affects job satisfaction and well-being but positively correlates with burnout. Conversely, life-work interference positively influences job satisfaction but negatively impacts work engagement. Both work engagement and job satisfaction positively affect well-being, while burnout shows a negative relationship. Notably, work-life time balance positively influences job satisfaction and well-being, and overtime work has a surprisingly positive effect on these aspects. This research contributes to existing literature by underscoring the distinct experiences of the self-employed in the digital age, laying a groundwork for future research.

PLOS ONE

Comparative impact of insect growth regulators on mortality and development of <i>Amrasca biguttula</i> (Hemiptera: Cicadellidae)

by Sabrine Attia, Shimat V. Joseph The two-spot cotton leafhopper, Amrasca biguttula (Ishida) (Hemiptera: Cicadellidae), recently detected in the United States, represents an emerging threat to cotton, vegetable, and ornamental crops. Insect growth regulators (IGRs) are considered reduced-risk insecticides. Despite their availability to growers and effectiveness on several piercing and sucking insects, the lethal effects of IGRs on the development of A. biguttula remain poorly understood. Thus, the objective of this study was to determine the effects of common IGRs on various stages of A. biguttula. We evaluated four IGRs: pyriproxyfen, novaluron, azadirachtin, and buprofezin applied at field-recommended rates, alone or combined with nonionic and organosilicone adjuvants, on survival, molting disruption (exuviae production), and longevity of early (1st-2nd), intermediate (3rd-4th), and late (5th) nymphal instars, as well as adults using leaf dip and adaxial leaf smear bioassays. All IGRs induced significant, stage-dependent lethal effects. Mortality of 1st–2nd instars reached over 90% with buprofezin and novaluron, and molting inhibition reached up to 55%, indicating strong effects of the tested insecticides. The chitin biosynthesis inhibitors buprofezin and novaluron caused rapid mortality, strong molting inhibition, and reduced longevity, particularly in early and intermediate instars. Pyriproxyfen and azadirachtin elicited weaker, delayed responses, with limited effects on late instars and adults. Although adding adjuvants slightly enhanced efficacy, their overall impact was marginal. These findings demonstrate that IGRs can profoundly disrupt A. biguttula population development through interference with insect growth and metamorphosis, supporting their use as selective and sustainable tools in integrated pest management programs targeting this invasive leafhopper.

PLOS ONE

The application of large language models in bariatric surgery: A scoping review

by Ningjing Guo, Xuyan Li, Xiaoxue Li, Congmin Kang, Xiaoyan Gong, Xinyu Ji, Jie Zheng Background Exploratory applications of large language models within the specialized field of metabolic and bariatric surgery have begun to emerge. Nevertheless, existing research remains fragmented, lacking comprehensive integration. Objective To conduct a scoping review of studies on the application of large language models in the field of metabolic and bariatric surgery, aiming to provide a reference for clinical practice and future research. Methods This scoping review adhered to the Joanna Briggs Institute methodological framework and followed the preferred reporting items for systematic reviews and meta-Analyses extension for scoping reviews (PRISMA-ScR) guidelines.PubMed, Web of Science, The Cochrane Library, Embase, CINAHL, CNKI, Wanfang, and VIP databases were searched for relevant studies, with the search timeframe from database inception to November 2025. The included literature was summarized and analyzed. Results A total of 21 English-language studies were included. LLMs were primarily applied in scenarios such as patient education and information consultation, clinical decision support, and professional knowledge assessment. While LLMs performed well in information-provision tasks, they showed low consistency with expert opinions in complex clinical tasks such as individualized surgical recommendations. Performance varied across different models, with GPT-4 generally demonstrating superior performance, and domain-specific models showing professional potential. Current research still faces challenges regarding information accuracy, readability, and clinical applicability. Conclusion Large language models hold auxiliary potential in the field of metabolic and bariatric surgery, particularly for knowledge dissemination and patient education. However, their reliability in complex clinical decision-making remains limited. Future efforts should focus on conducting high-quality studies, advancing model specialization and standardized evaluation, and exploring safe and effective human-AI collaboration models.

PLOS ONE

Clinical performance of the BioFire Blood Culture Identification 2 panel for microorganism species identification and resistance gene detection in blood culture-positive specimens

by Haruki Naruse, Noriyuki Watanabe, Sachie Koyama, Sachi Tanaka, Yoshitada Taji, Yasuhiro Ebihara Introduction Bloodstream infections are life-threatening, and the rapid identification of pathogens and resistance genes is essential for the administration of appropriate antimicrobial agents. The BioFire Blood Culture Identification 2 (BCID2) panel on the FilmArray multi-parameter genetic analyzer is a fully automated PCR test that rapidly identifies species and resistance genes. Here, we compared the performance of the Filmarray BCID2 panel (BCID2 method) with the conventional method. Methods Among the blood culture-positive specimens submitted between January 2023 and November 2024, this study analyzed 201 specimens that contained the target microorganisms of the BCID2 panel. In our laboratory, after subculturing the culture medium obtained from a positive blood-culture bottle, we perform species identification using mass spectrometry and drug susceptibility testing (the conventional method). We compared the results of the BCID2 method with those of the conventional method. Results Concordance between the BCID2 and conventional methods was found in 152 of the 161 monomicrobial specimens (94.4%) and in 31 of the 40 polymicrobial specimens (77.5%). The 18 specimens that were discordant were mostly matched at the genus level, but the BCID2 method also detected other microorganisms that were not identified by the conventional method. Resistance genes were identified in 57 of the 61 matched specimens (93.4%). Conclusion The BCID2 method exhibits excellent identification results and resistance gene detection rates, suggesting that it is a reliable and rapid diagnostic test system for bloodstream infections.

PLOS ONE

Identifying key factors in building fires: A novel approach fusing K-shell entropy gravity

by Yongping Yu, Ning Wang, Shibo Cui, Enhui Zhao Building fire key factors are the fundamental control variables that govern both the initiation of fires and dynamics of propagation. The accurate identification of key factors in building fires is crucial for enhancing the effectiveness of fire prevention strategies. To improve the accuracy of key factor identification in building fires, a novel K-shell Entropy Gravity (KEG) algorithm that integrates multiple topological metrics is proposed in this study. First, a complex network is constructed to characterize the relationships among accident factors, where nodes represent influencing factors and edges denote their co-occurrence in fire incidents. Subsequently, considering the positional importance and core connectivity of nodes, the information influence and irreplaceability of nodes, as well as the collaborative coupling and nonlinear characteristic among multiple indicators, a composite attribute integrating K-shell value, information entropy difference, and total shortest path length is developed to quantify node importance, thereby capturing both the local coreness and the global influence of nodes within the network. Then, these metrics are incorporated into an established gravity-based model to comprehensively assess the influential scope of each node, and the results are employed to identify the key factors. Finally, the proposed method is compared with baseline methods based on the Susceptible–Infected–Recovered (SIR) model and network robustness evaluation using the California Building Fire Dataset (2012–2024). In addition, a sensitivity analysis is performed to investigate how the removal of key factors affects accident propagation. To further verify the robustness of this method, fire data from Alaska are applied for comparison, and an ablation experiment is designed. The results indicate that the KEG algorithm achieves superior accuracy in identifying critical factors and offers a reliable analytical tool for developing targeted fire prevention and mitigation strategies.

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