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전체arXiv CS.AI6,589arXiv Math6,552arXiv Physics2,334arXiv Stat1,126PLOS ONE453arXiv Econ323arXiv Q-Bio318eLife100PLOS Global Public Health90PLOS Biology54PLOS Medicine44
PLOS ONE

Study on tensile mechanical response and microstructure of polypropylene fiber reinforced loess under freezing

by Yuxing Wang, Chunhui Liu, Yimin Zhong, Erqing Mao, Fei He Tensile strength is one of the key parameters in the mechanics of frozen ground, widely applied in the engineering design of frozen ground and theoretical research on frost heave. Fiber-reinforced soil technology has attracted considerable attention from numerous researchers. This study utilised a custom-designed tensile testing apparatus to investigate the influence of polypropylene fiber content, freezing temperature, and loading rate on the mechanical response of frozen loess. Combined with microscopic structural observations, the research elucidates the fiber reinforcement mechanism. Experiments revealed that fiber incorporation transformed the soil’s failure mode from brittle fracture to ductile failure, characterised by multiple fissures, significantly enhancing the material’s load-bearing capacity and deformation properties. Optimal reinforcement was achieved at a fiber content of 0.3%, with tensile strength increasing by 71.4% compared to unfibered soil. The stress-strain response exhibited sustained strengthening characteristics. Tensile strength exhibits exponential growth with decreasing freezing temperatures and linear increase with rising loading rates. The incorporation of fibers further enhances the material’s response capability under dynamic loading conditions. Microstructural analysis indicates that an appropriate fiber content effectively fills soil pores and forms a spatial network structure, whereas excessive fibers cause agglomeration due to uneven distribution, resulting in structural weakening. This research provides experimental evidence and theoretical reference for the design and application of fiber-modified frozen ground in engineering projects within cold regions

PLOS ONE

Biomedical engineer density per hospital bed: A medical device quality indicator for Mexican Healthcare System

by Luis G. Ayala, Nadezhda Aguilar Blas, Norma Patricia Navor Galeana Background The measurement of health indicators that enable the estimation of a system’s capacity to provide adequate patient care constitutes a highly relevant factor in the evaluation of healthcare systems. Traditionally, these indicators include variables such as physician density, nursing staff, and other human resources; however, a critical component that often receives less attention is the capacity of healthcare institutions to sustain high-quality infrastructure and medical technology, which are essential to ensuring the continuity, safety, and efficiency of healthcare services. In this context, an important complementary metric would be the density of biomedical engineers within hospital settings, as these professionals are required to design, evaluate, regulate, acquire, maintain, and manage medical technologies and healthcare infrastructure. Current WHO metrics measure biomedical engineer density per 10,000 inhabitants, not reflecting their direct relationship with medical devices and hospital infrastructure. Objective To develop a novel biomedical engineer density indicator per hospital beds (ρBEhb) that relates workforce capacity to medical devices rather than general population, and to evaluate its applicability within the Mexican healthcare system. Methods The ρBEhb calculates the ratio between biomedical engineers providing professional services (internal or mixed staff) and available hospital beds for a given year. The methodological framework follows PAHO/WHO ‘Health Indicators’ guidelines. Data from the Mexican healthcare system (2017–2022) were analyzed alongside international benchmarks. Results The Mexican healthcare system showed generally positive ρBEhb trends from 2017 to 2022, with a −0.53% decrease (2017−2018) and a notable 14.41% increase (2020−2021) coinciding with the SARS-CoV-2 pandemic. While Mexico's absolute number of biomedical engineers is comparable to the USA, ρBEhb analysis reveals a dual structural deficit — insufficient hospital beds and inadequate biomedical engineering workforce — when adjusted for population size and international standards. Conclusions The ρBEhb indicator exposes Mexico's dual deficit in hospital beds and biomedical engineers relative to population needs. This metric provides a more relevant measure for assessing medical device management capacity and healthcare quality than traditional population-based indicators.

PLOS ONE

Embodied intelligence-driven adaptive collaboration in supply chains: A four-dimensional synergy framework and mechanism analysis

by Ziqiao Ding, Hanjiang Lin, Huiying Xu, Xiaolei Zhang, Xinzhong Zhu Existing research focuses on data-driven algorithm optimization but overlooks the embodied nature of supply chains as physical and digital integrated systems, leading to a disconnect between AI and physical collaboration. This study introduces embodied intelligence into supply chain management, transcending the traditional paradigm to propose an adaptive collaboration framework through embodied perception, contextual reasoning, and physical execution. It deconstructs the core of supply chain embodied intelligence, revealing issues such as fragmented perception and delayed feedback. Based on embodied cognition and complex adaptive systems theory, a four-layer architecture with embodied perception, contextual reasoning, physical execution, and closed-loop feedback is constructed, clarifying its mechanisms. Future directions in theory, technology, and practice are outlined. This work deepens the integration of embodied intelligence with supply chains, bridges the digital and physical divide, and advances supply chain management toward an embodied adaptive paradigm for next-generation intelligent systems.

PLOS ONE

Promoting shared decision-making in colorectal cancer screening in primary care: A cluster randomized controlled trial

by Tamara Scharf, Yonas Martin, Marc-Andrea Janggen, Adrian Rohrbasser, Kali Tal, Nikola Biller-Andorno, Jean-Luc Bulliard, Kevin Selby, Reto Auer Introduction In Switzerland, primary care physicians (PCP) prescribe colonoscopy for colorectal cancer (CRC) screening rather than offering a choice between colonoscopy and faecal occult blood test (FOBT). This study evaluated a training program promoting shared decision-making for CRC screening. Methods PCP from a research network were randomized 1:1 into intervention or control. The intervention group received study materials, patient decision aids, evidence summary, FOBT sample kit, and personalized feedback on CRC screening practices. PCP documented CRC screening decisions of 40 consecutive patients (ages 50–75) four months post-intervention. The control group received no materials before data collection. Results Of 110 PCP randomized, 83 (76%) collected data on 3,171 patients (mean age 62, 50% women). PCP in the intervention group were more likely than controls to have at least one patient tested or planning FOBT (84% vs. 56%; unadjusted RR: 1.52; 95% CI: 1.13 to 2.04). In a sensitivity analysis restricted to 62 PCP who participated in a previous data collection, 72% (21/29) already met the primary outcome in the intervention group at baseline and 49% (16/33) in the control group (RR: 1.49; 95% CI: 0.98 to 2.28). When contrasting the change within PCP from the 2017 and 2018 data collection, there was no significant increase in proportion of PCP who met primary outcome between intervention and control group, while it might have increased the proportion of PCP already prescribing FOBT to prescribe it to more of their patients. Conclusion A mailed intervention increased FOBT prescriptions, but selection bias may have influenced results.

PLOS ONE

Can heat stress affect the psychophysiological responses and locomotor demands of young soccer players during small-sided soccer games?

by Ersan Arslan, Yusuf Soylu, Ana Filipa Silva, Osman Yilmaz, Bulent Kilit, Neslihan Akçay, Lukasz Radziminski, Ladislav Batalik The study compared the effects of three different heat stress conditions on the psycho-physiological responses and locomotor demands of young players in different small-sided soccer games (SSGs). Sixteen soccer players (age: 16.5 ± 0.5 years) performed 2-a-side and 4-a-side SSGs under three environmental heat stress conditions: low environmental heat (LEH) ≤ 23.9°C, moderate environmental heat (MEH) 24.0–27.9°C, and high environmental heat (HEH) 28.0–32.9°C. Players’ heart rate (HR) responses and total distance covered (TDC) were continuously monitored for all SSGs; the rating of perceived exertion (RPE) and visual analog scale (VAS) were used after each bout. Tympanic temperature (TT) was recorded daily before and after the SSGs. The results demonstrated that for both 2-a-side and 4-a-side SSGs, significant main effects of temperature were observed for HR, %HRmax, RPE, and VAS responses (all p max, RPE, and VAS responses (all p < 0.05), suggesting that responses to heat stress varied across repeated bouts. However, no significant interaction effects were observed for TDC or TT in the 4-a-side SSGs, while only limited interaction effects emerged for TDC in the 2-a-side SSGs format. Overall, the findings indicate that heat stress substantially amplifies cardiovascular and perceptual responses during SSGs, with effects modulated by game format and bout structure. In contrast, TDC and TT appear less sensitive to these conditions. Coaches may use this evidence to manage players’ internal and external load and optimize their team’s performance across various heat-stress conditions.

PLOS ONE

Integrative computational analysis of plant-derived flavonoids as inhibitors of Listeriolysin O and Internalin A in <i>Listeria monocytogenes</i>

by Shahadul Hassan Sourav, Noimul Hasan Siddiquee, Rahnuma Tabassum, Lamia Islam, Syed Kashif Zaidi, Mohammad Sharif Uddin, Sumita Rani Saha, Imam Hossain Foodborne pathogen Listeria monocytogenes causes listeriosis, a rare but deadly condition. Internalin A (InlA) and Listeriolysin O (LLO), its main virulence factors, facilitate adhesion, invasion, intracellular survival, and intercellular spreading, making them interesting therapeutic targets. L. monocytogenes infections are becoming harder to treat because of antibiotic resistance; hence, flavonoids are being considered. An integrated in-silico technique was used to test plant-derived flavonoids’ inhibitory efficacy against these proteins. For both targets, three modes of docking (HTVS, SP, and XP docking) were used for the preliminary screening from a library of 1,254 flavonoids. While CIDs 441667, 15126294, and 187808 showed favorable in-silico profiles for InlA with scores of −8.461, −7.578, and −7.521 kcal/mol, respectively, CIDs 441699, 443648, and 442868 showed the best affinity for LLO with values of −7.446, −5.991, and −5.852 kcal/mol, respectively. Admet analysis predicted the drug-likeness and safety characteristics of the compounds. Subsequently, the QM calculation was employed to examine the interaction of these compounds with the receptor, alongside their MEP and NBO characteristics. The selected ligands and the control ampicillin for both proteins were utilized to build protein-ligand complexes, subsequently assessed via a 100 ns molecular dynamics simulation. Subsequent post-simulation MM-GBSA, PCA and DCCM analysis of the trajectories evaluated their dynamic stability concerning InlA and LLO. CIDs 441667 and 15126294 for InlA, as well as CIDs 441699 and 443648 for LLO, have been identified as potential inhibitors, establishing a basis for future in vivo investigations and experimental validation.

PLOS ONE

Replicative and stress-induced premature senescence distinctively affect the endothelial anticoagulation capacity

by Akiko Katayama, Koji Ikeda, Tomoya Kitani, Ekura Yamazaki, Daisuke Ueno, Fumiaki Ito, Satoaki Matoba Aging is strongly associated with an increased risk of morbidity and mortality from multiple diseases, including thromboembolic disorders. Endothelial dysfunction is considered a key contributor to age-related thrombus formation, although its underlying mechanisms remain incompletely understood. Cellular senescence is a fundamental driver of aging; however, the distinct functional roles of replicative (RS) and stress-induced premature senescence (SIPS) in cellular functions remain unclear. This study, investigated the effects of endothelial cell (EC) senescence on blood coagulation, and demonstrated that RS and SIPS differentially regulate endothelial anticoagulation capacity. Plasma coagulation capacity, assessed using calibrated automated thrombogram, was unexpectedly reduced in the presence of RS-ECs compared with that in the presence of young control cells, whereas SIPS-EC showed no such effect. RNA sequencing analysis revealed distinct global transcriptional and coagulation pathway-related alterations between RS- and SIPS-ECs. Despite enhanced anticoagulation capacity in RS-ECs in vitro, thrombus formation was exacerbated in naturally aged mice in vivo. The contribution of SIPS-ECs to thrombus formation was further evaluated in vivo using EC-specific SIPS mouse models. EC-specific SIPS mice exhibited aggravated venous thrombus formation, with thrombus histological features resembling those observed in naturally aged mice. Gene expression profiles related to blood coagulation were also largely similar between ECs isolated from naturally aged and EC-specific SIPS mice. These findings demonstrate distinct contributions of endothelial RS and SIPS to blood coagulation and suggest that SIPS, rather than RS, may represent the predominant form of endothelial senescence during in vivo aging with respect to age-related dysregulation of blood coagulation.

PLOS ONE

What do youth need to know about puberty? A scoping review protocol to identify puberty education competencies

by Marie A. Brault, Nanki Singh, Nikita Kakkad, Melissa Peskin, Anthony Betori, Gregory Laynor, Emily Naiser Introduction Puberty is a key transition point in adolescents’ lives that plays a foundational role in shaping health behaviors and outcomes across one’s life course. This period holds significant potential to empower adolescents and support autonomy in health and well-being, but limited puberty education curricula exist for early adolescents (age 8–14), and those that do exist vary in content. There is a paucity of evaluations of puberty competencies and limited consensus on what competencies should be measured to assess effectiveness or even how to measure these competencies. Objective The objective of this scoping review is to systematically map and characterize the outcomes, domains, and instruments used to evaluate puberty education curricula for early adolescents aged 8–14 years. In accordance with PRISMA-ScR and JBI scoping review guidance, this review does not synthesize effect sizes or assess intervention efficacy, but maps the breadth of evidence to identify conceptual gaps and inform future framework development. Methods The review protocol is registered with the Open Science Framework (OSF). We will search PubMed, CINAHL, PsycInfo, ERIC, Education Source, Scopus, Web of Science Core Collection, ProQuest Dissertations and Theses Global, and OpenAlex for relevant sources. Two reviewers will independently screen and extract studies that meet inclusion criteria using our data extraction tool. Expected outputs Findings from the scoping review will be synthesized to create an overarching framework that can guide approaches to the development and evaluation of puberty curricula targeted to early adolescents. Focus group discussions with adolescents, parents, and school representatives will be conducted to assess the applicability and appropriateness of identified competencies and evaluation measures prior to broader dissemination. Insights from this scoping review will ultimately be used to inform the implementation and evaluation of puberty education.

PLOS ONE

Mechanical properties and energy evolution characteristics of fissure sandstone under the interaction between water and fissures

by Qingqing He, Jianhua Deng, Guixin Yuan To investigate the mechanical properties and damage evolution of fissure sandstone under the interaction between water and fissures, this study performed uniaxial compression tests on sandstone specimens with different water conditions (dry, natural, and saturated) and fissure angles (0°, 30°, 45°, 60°, and 90°). The experimental results indicate that peak strength decreased markedly with increasing water content, with reductions of 28.68%–53.99% under saturated conditions relative to dry conditions. In contrast, peak strength increased progressively with fissure angle. Crack initiation stress and crack damage stress exhibited similar trends. Based on the normalized ratios of characteristic stress, two damage evaluation indices, Rci and Rcd , were proposed to characterize the weakening effect of fissures on rock bearing capacity during the crack initiation and crack propagation stages, respectively. The energy evolution results show that the strain energy corresponding to characteristic stress decreases significantly with increasing water content and generally increases with fissure angle. In addition, this study introduced a warning coefficient λ based on the ratio of elastic strain energy to dissipated strain energy to identify precursor information associated with rock failure. The results show that λ increased with water content and varied with fissure angle in an M-shaped pattern, with significant peaks at 30° and 60°. Under saturated conditions, water exerted the strongest effect on mechanical parameters at a fissure angle of 0°, while the overall effect remained relatively small at 30°. These findings provide a valuable reference for risk assessment and disaster prevention in geotechnical engineering.

PLOS ONE

Expression of Concern: Computational modeling and analysis of medical resource shortages in hospital alliance: A simulation-driven approach

by The PLOS One Editors

PLOS ONE

Expression of Concern: ING5 is phosphorylated by CDK2 and controls cell proliferation independently of p53

by The PLOS One Editors

PLOS ONE

Early mortality risk stratification in childhood bacterial meningitis using cerebrospinal fluid glucose and protein levels and their combinations: A multicontinental cohort study

by Markku Kallio, Tuula Pelkonen, Irmeli Roine, Heikki Peltola Background Bacterial meningitis remains a major cause of death and neurological disability in children worldwide, particularly in low-resource settings where access to intensive care is limited. Cerebrospinal fluid (CSF) glucose, protein, and leukocyte concentrations are routinely measured at hospital presentation, yet their potential for early risk stratification – individually or in combination – has not been clearly defined. Because mortality risk is often difficult to assess at admission, identifying simple CSF-based thresholds that help clinicians recognize high-risk patients could improve triage and management globally. Methods and findings We evaluated CSF glucose, protein and leukocyte levels in 1598 children with bacteriologically confirmed bacterial meningitis across six countries in Latin America, Angola, and Finland to determine their individual and combined associations with mortality and neurological outcome. CSF glucose, protein, and leukocyte counts were measured at admission and examined in relation to in-hospital mortality and neurological outcome at discharge, as assessed by the Glasgow Outcome Scale. CSF glucose and protein concentrations were categorized into five clinically interpretable groups. Mortality increased progressively with decreasing CSF glucose (from 8% to 29%) and with increasing protein concentrations (from 5% to 23%). Children with both low CSF glucose (<10 mg/dL) and high CSF protein (≥200 mg/dL) had a mortality rate of 27% compared with 1% among those with normal CSF glucose and protein. A CSF leukocyte count <500/µL was also associated with increased mortality. Lower CSF glucose (ρ = 0.24; P < .001) and higher protein (ρ = 0.15; P < .001) were both associated with poorer neurological outcomes. Conclusions Routine CSF markers available at the time of hospital admission provide prognostic information that supports risk stratification in pediatric bacterial meningitis. Simple CSF-based thresholds—particularly when CSF glucose and protein are interpreted in combination—may help identify high-risk children early and guide clinical prioritization in settings with limited critical care capacity.

PLOS ONE

Correction: Evolving threats: Leveraging <i>C. elegans</i> to decode the virulence profiles of highly related environmental <i>Salmonella</i> Newport isolates

by Christina M. Ferreira, Mirae Choe, Bella Wayhs, Julie A. Haendiges, Robert Literman, Jianghong Meng, Arjuman Ghazi, Rebecca L. Bell

PLOS ONE

Expression of Concern: A modified fractional short circuit current MPPT and multicellular converter for improving power quality and efficiency in PV chain

by The PLOS One Editors

PLOS ONE

Correction: Better start to bilingual development: Bridging parental beliefs and science through early intervention for Polish families living in Norway

by The PLOS One Staff

PLOS ONE

Metabolite profiling of saliva for the discrimination of Behcet’s disease, Sjögren’s syndrome, and recurrent aphthous stomatitis

by Sooah Kim, Ming Zhang, Joong Kyong Ahn, Jiwon Hwang Oral manifestations can be the initial sign of systemic diseases such as Behcet’s disease (BD) and Sjogren’s syndrome (PSS). Their frequency and morphology vary widely, and recurrent aphthous stomatitis (RAS) often mimics BD ulcers, complicating differentiation on clinical grounds. Nonspecific aphthoid lesions are also seen in PSS. This study aimed to identify distinctive metabolic patterns in saliva that could discriminate BD, PSS, and RAS using global metabolite profiling. Saliva samples were collected from 43 patients (BD, n = 24; PSS, n = 10; RAS, n = 9) after fasting and abstaining from oral activities for at least 90 minutes. Gas chromatography-mass spectrometry (GC/MS) was employed for metabolite profiling. Principal component analysis (PCA) and hierarchical clustering analysis (HCA) were used to distinguish groups. Variable importance in projection (VIP) scores were calculated from partial least squares discriminant analysis (PLS-DA), and ANOVA was applied to compare metabolite abundance. Forty-two metabolites were identified and categorized into amino acids, organic acids, sugars, sugar alcohols, and others. PCA and HCA demonstrated clear dis-crimination among BD, PSS, and RAS. Metabolites with VIP > 1 included malonic acid sorbitol, pyroglutamic acid, aspartic acid, decanoic acid, hexadecenoic acid, and L-proline. Notably, malonic acid and aspartic acid were significantly elevated in BD compared to PSS and RAS, suggesting their diagnostic potential. Global salivary metabolite profiling by GC/MS provides distinct metabolic signatures enabling discrimination among BD, PSS, and RAS. This approach may enhance understanding of oral mucosal manifestations in systemic autoimmune diseases.

PLOS ONE

Correction: Prediction of the ectasia screening index from raw Casia2 volume data for keratoconus identification by using convolutional neural networks

by Maziar Mirsalehi, Benjamin Fassbind, Andreas Streich, Achim Langenbucher

PLOS ONE

Correction: The structure of spontaneous speech changes in Alzheimer’s disease: Crosslingual evidence from English and Greek

by Hong Jiang, Zhengwei Chen, Yu Liu, Chun Yang, Xiaofeng Yuan, Rui He

PLOS ONE

Longitudinal associations between perceived benefits and costs of internet gaming and internet gaming disorder in adolescent gamers: A cross‑lagged structural equation model

by Xue Yang, Xin Wang, Miguel Ribeiro Ramos This longitudinal panel study aims to investigate the relationships between short/long-term benefits and costs of internet gaming and internet gaming disorder (IGD) in adolescents. First-year high school students with a convenience sampling were recruited from four high schools in central China, and 1032 (56% boys) finished baseline and one-year follow-up surveys. The percentage of IGD was 14.8% at T1 and 13.9% at T2. Cross-lagged structural equation modeling showed that higher levels of IGD symptoms predicted more perceived short- and long-term costs of gaming, while perceptions of short- and long-term costs or benefits did not significantly predict IGD symptoms. These findings suggest experiencing actual negative consequences of internet gaming could enhance perceived costs. Prospective associations between cognitive perceptions and IGD are highlighted and the implications for interventions are discussed.

PLOS ONE

Risk factors for fecal incontinence after surgery for cryptoglandular anal fistula: Protocol of a meta-analytic study

by Cheng Tang, Zubing Mei Introduction Fecal incontinence after anal fistula surgery is a feared functional complication that can impair dignity, social participation, and quality of life. Reported rates vary widely across procedures and fistula phenotypes, and individual studies suggest multiple patient-, disease-, and surgery-related predictors. However, the prognostic evidence remains dispersed, with inconsistent outcome definitions and variable adjustment for confounding. This protocol describes a systematic review and quantitative synthesis to identify factors associated with postoperative fecal incontinence after surgery for cryptoglandular anal fistula. Methods and analysis The study will follow the Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols guidance. PubMed, Embase, Cochrane Library, Web of Science, and grey-literature sources will be searched from inception to January 11, 2026, without language restrictions. Eligible studies will be cohort or case-control studies of adults undergoing surgery for cryptoglandular anal fistula that report new-onset or worsening postoperative fecal incontinence using validated scores or clearly defined clinical criteria at least 3 months after surgery. Two reviewers will independently screen records, extract data, and assess risk of bias using the Quality in Prognosis Studies tool and, where applicable, the Newcastle-Ottawa Scale. Adjusted estimates will be prioritized for random-effects meta-analysis when studies are clinically comparable. Heterogeneity and confounding will be explored by fistula complexity, procedure type, follow-up duration, and baseline continence assessment. Certainty of evidence will be rated using a prognostic-factor approach. Discussion By integrating observational evidence across diverse surgical strategies, this review aims to generate pooled, evidence-based prognostic estimates for postoperative fecal incontinence. The findings may support preoperative counseling, baseline continence assessment, individualized procedure selection, sphincter imaging where appropriate, and targeted strategies to reduce preventable functional harm. Ethics and Dissemination No ethical approval is required as this study uses existing data. Results will be disseminated through peer-reviewed journals and conferences to inform anal fistula management. Registration International Prospective Register of Systematic Reviews (CRD420261283433)

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