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PLOS ONE

Longitudinal association of quality of life and health behaviors with service member readiness

by Yunnuo Zhu, Sheila F. Castañeda, Isabel G. Jacobson, Crystal L. Lewis, Felicia R. Carey, Scott C. Roesch, Rudolph P. Rull, Millennium Cohort Study Team Background This study aimed to assess whether self-reported physical and mental health-related quality of life (HRQOL) scores could be useful indicators of military health readiness. Methods Survey data and Department of Defense administrative and medical records from 51,589 active duty Millennium Cohort Study participants across four enrollment panels (2001, 2004, 2007, 2011) were examined at baseline and 3- to 5- year follow-up endpoints. Baseline measures included HRQOL measured by the Veterans RAND 12-Item Health Survey (VR-12) mental and physical component summary scores, health behaviors (e.g., smoking, insomnia symptoms), and demographic and military covariates. Readiness outcomes measured at follow-up included health care utilization, separation status, military satisfaction, lost workdays, and non-obese body mass index (BMI). Poisson regression models with a robust error variance were used to examine the prospective relationship between HRQOL and military readiness outcomes. Results HRQOL remained a significant predictor of all five readiness outcomes after adjustment for covariates and other health behaviors. Participants with baseline physical HRQOL scores in the highest 15th percentile were more likely to meet readiness metrics (e.g., increased odds of non-obese BMI, fewer annual lost workdays, less annual health care utilization, and a higher likelihood of remaining in service or completing their service term) compared with those in the middle 70th percentile. Scoring in the lowest 15th percentile for baseline physical or mental HRQOL was associated with being less likely to meet readiness metrics 3–5 years later. Conclusion Higher physical HRQOL may be a positive indicator of military readiness, while lower physical and mental HRQOL may signal impaired readiness. Assessment of HRQOL using the VR-12 is an easy, patient-centered tool that may help military leaders identify service members needing early interventions and improve readiness and retention.

PLOS ONE

Human papillomavirus genotypes and factors associated with major cervical smear abnormalities in a sickle cell endemic area of Kisangani, Democratic Republic of the Congo

by Yvette Neema Ufoy Mungu, Joëlle Desreux, Elettra Bianchi, Burubu Lisi-Ankiene Junior, Roland Marini Djang’eing’a, Durkin Keith, Artesi Maria, Katenga Bosunga, Vincent Bours Background Cervical cancer due to high-risk human papillomavirus (HR-HPV) is the leading gynecological cancer in Sub-Saharan Africa and in the Democratic Republic of the Congo (DRC), where approximately one-quarter of people carries the sickle cell allele. This study aims to identify the HR-HPV genotypes and factors associated with major cervical abnormalities among women living in the sickle cell endemic area of Kisangani, DRC. Methods This hospital-based cross-sectional study consisted to a face-to-face and semi-structured questionnaire for collecting data of the socio-demographic, clinical and sexual behaviors characteristics of participants. The sickle cell status was confirmed using liquid chromatography coupled with mass spectrometry. Cervical smears were collected using the ThinPrep® Pap-test solution (Hologic Inc, Marlborough, USA). The HR-HPV molecular analysis was performed using the Cobas® 6800 system (Roche Molecular Systems, Somerville, USA) for real time polymerase chain reaction (RT-PCR) and the Oxford Nanopore platform (Oxford Nanopore Technologies, Oxford, England) for the viral genome sequencing. Cytological analyses were performed using the ThinPrep 5000 processor (Hologic), and the results were reported according to the 2014 Bethesda System classification. The logistic regression model was used to estimate the adjusted Odd ratio (aOR) during the risk factor assessments. Results Among 712 women enrolled, the prevalence of HR-HPV was 28.4% (95% CI: 25.1–31.7). HPV35, HPV52 and HPV31 were the most common genotypes; while the sequencing identified two new variants of HPV103 and HPV223. The following factors were associated with HR-HPV infection: (i) age between 25 and 34 years (aOR: 2.10, 95% CI:1.22–3.69); (ii) being unmarried (aOR: 1.54, 95% CI: 1.05–2.27) and (iii) HIV-positive status (aOR: 14.85, 95% CI: 2.36–288.25). No correlation was observed between hemoglobin AS (HbAS) status and HR-HPV infection or cytological abnormalities. However, women aged 45–54 years (aOR: 3.8, 95% CI: 1.34–11.65), those using intravaginal herbal (aOR: 3.59, 95% CI: 1.26–11.28) and those infected with HPV33 (aOR: 8.45, 95% CI: 1.68–34.05) were more likely to present major cervical abnormalities in our series. Conclusion Our study shows a high prevalence of cervical HR-HPV infection in Kisangani, DRC. HPV35, HPV52, and HPV31 are the three most common genotypes, while novel variants of HPV types 103 and 223 were identified. Our findings also highlight that advanced age, HPV33 infection and intravaginal herbal use are the main factors associated with major cervical abnormalities. Although HbAS has not been associated with HR-HPV infection or cervical lesions, further studies are needed to determine this association in women with sickle cell disease (HbSS).

PLOS ONE

Coordinate aware implicit neural representation for UAV small object detection

by Yong Yang, Tianci Wan, Ling Guo, Menglu Zhang Small object detection in unmanned aerial vehicle (UAV) imagery remains challenging due to low resolution, complex backgrounds, and limited pixel occupancy. Conventional CNN-based detectors rely on fixed receptive fields, which often fail to capture fine-grained spatial details and implicit geometric relationships. To address these limitations, this paper proposes a Coordinate-Aware Implicit Neural Representation Enhanced C2INR module that integrates coordinate-based representations into convolutional feature extraction. The Multi-Scale Coordinate Encoder (MSCE) constructs frequency-aware positional embeddings through sinusoidal encoding to enhance spatial continuity at multiple scales. The INR Feature Enhancer (IFE) further fuses encoded coordinates with visual features via lightweight MLP modulation, improving sensitivity to small-scale variations. Additionally, a Small Object Attention mechanism combines global context, local detail, and high-frequency cues to strengthen responses to tiny targets. Experiments on three UAV benchmarks—AI-TOD, UAVDT, and VisDrone—demonstrate consistent improvements over existing methods with minimal computational overhead. Further evaluation on PASCAL VOC verifies strong cross-domain generalization. These findings confirm that coordinate-aware implicit representation provides an effective and broadly applicable solution for improving spatial continuity, geometric fidelity, and localization precision in small-object detection.

PLOS ONE

Phytochemical composition, antioxidant potential, and enzyme inhibitory properties of <i>Onosma thracica</i> extracts: A comparative study of extraction methods

by Mamdouh Alshammari, Cengiz Sarikurkcu, Fevzi Bardakci, Mousa Alreshidi, Mohd Adnan, Riadh Badraoui, Bektas Tepe Onosma thracica is a promising natural source of bioactive phenolic compounds, including flavonoids, yet the influence of extraction technique on its chemical profile and biological properties remains unclear. In this study, methanol extracts prepared by maceration (MAC), ultrasound-assisted extraction (UAE), and Soxhlet extraction (SOE) were comparatively investigated in terms of their detailed phenolic composition, antioxidant capacity, enzyme inhibitory activity, and in silico interaction patterns of individual compounds. Among the tested methods, UAE yielded the highest total phenolic content (45.41 mg GAEs/g extract), whereas SOE produced the richest flavonoid content (71.05 mg REs/g extract). LC–ESI–MS/MS analysis showed that luteolin-7-glucoside and apigenin-7-glucoside were the dominant flavonoids, while chlorogenic acid and rosmarinic acid were the major phenolic acids. Biological assays revealed method-dependent differences in activity. UAE was more effective in total antioxidant capacity and reducing power, while SOE showed stronger radical scavenging and metal chelating performance. In enzyme inhibition assays, SOE displayed the strongest acetylcholinesterase inhibition, whereas tyrosinase inhibition was similar across the extracts. Correlation analysis indicated that the observed bioactivities were closely associated with the overall phenolic composition (including flavonoid constituents), particularly with luteolin-7-glucoside and rosmarinic acid. Docking studies showed favorable interactions of apigenin-7-glucoside and luteolin-7-glucoside with human α-amylase, apigenin-7-glucoside and luteolin with acetylcholinesterase, and rosmarinic acid with human tyrosinase-related protein 1, suggesting that these major phenolic constituents may contribute to the observed bioactivities of O. thracica. The results demonstrate that extraction method markedly shapes the phytochemical composition and bioactivity of O. thracica, and they support further work aimed at optimizing its potential pharmacological use.

PLOS ONE

Barriers and facilitators to healthcare access among Sub-Saharan African migrants in Europe: A scoping review

by Adanze Nge Cynthia, Jude Tsafack Zefack, Faustus Ajamah, Selouis Fuanyi Nkengfua, Emmanuel Mouladje Tchuela, Esua Alphonsius. F Background Sub-Saharan African (SSA) migrants represent a significant and growing proportion of Europe's diverse migrant population; yet face substantial barriers in accessing healthcare services. Understanding these barriers is essential for developing equitable healthcare policies and anticipating long-term care needs as these populations age. Methods This scoping review systematically mapped and synthesised existing evidence on healthcare access, utilization, barriers, and facilitators among SSA migrants across European settings. The primary review question was: What are the barriers and facilitators to healthcare access and utilization among Sub-Saharan African migrants in Europe? Following Arksey and O'Malley framework and PRISMA-ScR guidelines, we systematically searched Europe PMC, Scopus, Web of Science, PubMed, and Google Scholar. Fourteen studies met inclusion criteria. Data were synthesised using thematic analysis to identify descriptive and analytical themes. Results Three overarching analytical themes emerged: (1) structural determinants as persistent barriers to care, including health insurance gaps, legal precarity, and administrative complexity; (2) intersectional vulnerabilities shaping healthcare experiences, particularly among undocumented migrants, women, and those living with HIV; and (3) cultural mediation and system misalignment, characterized by language barriers and inadequate cultural competency. Only one study specifically examined ageing and chronic care decision-making. Studies predominantly employed qualitative designs (n = 7) and cross-sectional surveys (n = 4). SSA migrants encounter multi-layered barriers that compound over time, with critical implications for health trajectories as populations age. Conclusion Findings underscore the urgent need for migrant-sensitive healthcare approaches that address structural inequities, enhance cultural competency, and integrate long-term care planning. Future research should prioritize longitudinal studies examining healthcare needs across the life course and policy evaluations of migrant-inclusive interventions.

PLOS ONE

Cost-effectiveness of semaglutide versus dulaglutide for Type 2 Diabetes in China: A Markov Model analysis

by Qiying Chen, Tianyu Chen, Weicheng Lin, Xi Chen Objective From the perspective of China’s basic medical insurance, to evaluate the cost-effectiveness of semaglutide versus dulaglutide for type 2 diabetes mellitus (T2DM) in China, informing clinical and health policy decisions. Methods This study employed a Markov model to simulate the disease progression of T2DM over a 25-year time horizon. Transition probabilities between health states were sourced from the UK Prospective Diabetes Study Outcomes Model (UKPDS 82) and the SUSTAIN 7 clinical trial, while health utility values were obtained from published literature using the Chinese EQ-5D-5L value set. Long-term cost-effectiveness was projected through cohort simulation, with both one-way sensitivity analysis and probabilistic sensitivity analysis (PSA) conducted to evaluate the robustness of the model. Results After 25 years of simulation, the total cost for dulaglutide was ¥193,353.07, with 11.628 quality-adjusted life-years (QALYs) gained. The total cost for semaglutide was ¥240,925.08, yielding 11.900 QALYs. Compared with dulaglutide, semaglutide had an incremental cost-effectiveness ratio (ICER) of ¥174,904 per QALY gained, approximately twice China’s 2023 per capita GDP (¥89,358). According to the Chinese Guidelines for Pharmacoeconomic Evaluation (2020), this result falls within the economically acceptable range (1 × GDP < ICER ≤ 3 × GDP). One-way sensitivity analysis showed that although health state utility values were the most influential parameter, the model results remained robust. PSA indicated that at a willingness-to-pay (WTP) threshold of 3 times the per capita GDP (¥268,074), semaglutide had an 89.6% probability of being considered economically acceptable. Conclusion From the perspective of China’s basic medical insurance, semaglutide demonstrates cost-acceptable outcomes compared to dulaglutide in managing type 2 diabetes. This analysis provides crucial evidence for clinical decision-making and health policy formulation.

PLOS ONE

Effectiveness of shortwave diathermy in patients with chronic low back pain: A study protocol for a randomised, single-blinded, multicentre clinical trial

by Mohammad Ali, Saddam Hossain, MD. Abu Bakar Siddiq, Monirul Islam Background Chronic low back pain (CLBP) is a leading cause of disability worldwide and a major public health concern in low- and middle-income countries such as Bangladesh. Although international guidelines classify shortwave diathermy as a low-value treatment, it remains widely used in physiotherapy practice. Evidence on the effectiveness of continuous shortwave diathermy (CSWD) for CLBP is limited and inconsistent. This study aims to determine whether CSWD, when combined with standard physiotherapy, provides additional benefits compared with sham CSWD plus physiotherapy. Methods This multicentre, randomised, single-blind, sham-controlled trial will recruit 208 participants aged 18–65 years with CLBP from tertiary hospitals and physiotherapy clinics in Dhaka, Bangladesh. Participants will be randomly allocated (1:1) to receive CSWD or sham CSWD, each combined with a standardised physiotherapy programme delivered three times weekly for four weeks. The intervention group will receive active CSWD (27.12 MHz, continuous mode, 20 min/session), while the control group will receive sham treatment. Primary outcomes include back pain intensity, leg pain intensity (0–10 numerical rating scale), and activity limitation (Oswestry Disability Index). Secondary outcomes include the Brief Pain Inventory, Global Rating of Change, Satisfaction with Treatment, DASS-21, Insomnia Severity Index, Treatment Credibility Questionnaire, and healthcare utilisation. Outcomes will be assessed at baseline, 4, 12, and 24 weeks. Data will be analysed using an intention-to-treat approach with linear mixed-effects models. Discussion This trial will provide high-quality, context-specific evidence on the clinical and cost-effectiveness of CSWD for CLBP in a resource-constrained setting. The findings will inform physiotherapy practice in Bangladesh and may guide the appropriate use or de-implementation of this commonly used modality. The rigorous sham-controlled design will also contribute to the broader evidence base on electrotherapy for chronic pain.

PLOS ONE

Multi-scale feature integration with enhanced cytomorph for high-accuracy cervical cytology classification

by Elif İlgazi Kılıç, Şafak Kılıç Accurate classification of cervical cytology images plays a crucial role in early detection and prevention of cervical cancer, which remains a significant global health challenge. Despite advancements in deep learning for medical image analysis, the unique characteristics of cervical cells, including subtle morphological differences and complex nuclear patterns, pose considerable challenges for automated classification systems. In this paper, we present a novel deep learning architecture specifically designed for cervical cytology image classification. Our approach integrates three key components: (1) a specialized data augmentation pipeline tailored for cytopathology images, (2) a Morphology Attention Module (MAM) that captures multi-scale cellular features with adaptive feature fusion, and (3) a Spatial-Channel Mixer (SCM) that efficiently encodes nuclear neighborhood spatial information. Extensive experiments on both the SIPaKMeD and Mendeley LBC datasets demonstrate the superior performance of our model, achieving state-of-the-art accuracy of 99.06% on the 5-class SIPaKMeD dataset and 98.55% on the Mendeley LBC dataset. Importantly, our approach reduces error rates by up to 82.5% compared to conventional CNN architectures and 61.8% compared to recent Vision Transformer approaches. The proposed architecture demonstrates robust generalization across different cell types and imaging conditions, making it a promising tool for enhancing cervical cancer screening programs. Our work contributes to the advancement of automated cytology analysis and has the potential to improve early detection of cervical abnormalities, particularly in resource-limited settings where expert cytopathologists may be scarce.

PLOS ONE

Clinical outcomes of critical limb ischemia in Buerger disease: A contemporary analysis from an academic referral center in Jordan

by Qusai Aljarrah, Mohamad Al-Badaineh, Hammam Bani Salman, Nizar R. Alwaqfi, Sohail Bakkar, Mahmoud Megdadi, Khalid A. Kheirallah, Mohammed Z. Allouh Objective Buerger disease (BD), thromboangiitis obliterans, is a rare occlusive peripheral arterial disease affecting young smokers. To date, no study has investigated the burden of this disease in Jordan. This study aimed to assess the clinical course and factors associated with adverse outcomes of BD patients with critical limb ischemia (CLI) in Jordan. Methods The medical records of all patients with BD who presented with CLI at King Abdullah University Hospital in Jordan between January 2015 and January 2024 were retrospectively retrieved. The epidemiological profile of retrieved cases was collected along with the course of treatment, adverse events, and outcome. Statistical analyses were performed to assess the relationships between patient presentation, treatment course, and outcome. Results The study comprised 41 BD patients with Rutherford categories (RC) (IV-VI). The majority were males (95.1%) in their third decade (51.2%). Most patients (92.7%) presented with ischemic ulcerations (RC V, VI). A multivariable regression model revealed that increased C-reactive protein levels (OR = 1.044, 95% CI [1.003, 1.087], p = 0.035), gangrene at presentation (RC VI) (OR = 11.545, 95% CI [1.444, 92.332], p = 0.021), and limb infections at admission (OR = 7.745, 95% CI [1.182, 50.755], p = 0.033) were practical predictors of ending up with major limb amputation. Conclusion In Jordan, BD is associated with a high rate of amputation, which imposes considerable financial and social burdens. Prompt recognition, early management, and stringent smoking cessation measures are imperative to slowing disease progression.

PLOS ONE

Mumtathil: Automatic PDPL compliance identification system of Arabic privacy policies documents

by Malak Mashaabi, Hend Al-Khalifa In the digital era, the extensive collection and use of personal data by websites and applications has prompted various countries to implement data protection laws. The Kingdom of Saudi Arabia introduced the Personal Data Protection Law (PDPL) to regulate the handling of personal data. However, manually assessing compliance with these laws is time-consuming, and costly. This paper presents an automated system named Mumtathil (مُمتثِل) that evaluates the compliance of Arabic privacy policies with the PDPL using multi-label classification (MLC) techniques. Our experiments demonstrated that the Support Vector Machine (SVM) outperformed other machine learning models and transformer-based models like CamelBERT and AraBERT, achieving an F-score of 92%. The system addresses dataset imbalance by augmenting the Saudi Privacy Policy Dataset with recent privacy policies and synthetic data generated using ChatGPT. The Mumtathil tool simplifies compliance assessment, reducing time, cost, and effort while enhancing transparency and data protection in the digital landscape.

PLOS ONE

Cost analysis of a nationwide typhoid conjugate vaccine campaign in Burkina Faso

by Jean-Louis Koulidiati, Robert L. Zoma, Eric I. Nebié, Soumaila Yameogo, Christelle Neya Ouedraogo, Joël Arthur Kiendrébéogo, Frédéric Debellut Background In Burkina Faso, typhoid fever remains a major public health concern, particularly among children under 15. In January 2025, a nationwide campaign introduced the typhoid conjugate vaccine targeting children aged 9 months to 14 years. This study aimed to estimate the cost of typhoid conjugate vaccine delivery during the national campaign and to identify the main cost drivers across different administrative levels. Methods We conducted a retrospective cross-sectional costing study using a microcosting approach from the perspective of the Ministry of Health. We collected data from fifty health facilities, eight health districts, five health regions, and the national level. Financial and economic costs were estimated for each level, excluding vaccine and syringe costs. All costs were converted to 2024 USD using the official exchange rate. Parameter uncertainty was explored through one-way sensitivity analysis on key assumptions. Findings Vaccinators administered a total of 10.5 million typhoid conjugate vaccine doses. The average financial cost per dose was $0.47 (95% CI: $0.39–$0.51), and the economic cost was $2.16 (95% CI: $1.71–$2.56). Human resources and per diem payments were the main contributors to costs. Costs varied by geography, delivery strategy, and security context, with higher costs observed in rural and conflict-affected areas. The mobile–temporary posts strategy had the highest economic cost per dose ($2.02; 95% CI: $1.64–$2.40), while the fixed strategy had the highest financial cost per dose ($0.41; 95% CI: ($0.32–$0.49). Sensitivity analyses indicated robust cost estimates, with volunteer time valuation having the greatest influence. Conclusion The financial cost per dose remained within Gavi’s operational support range. The observed cost variations highlight the need for targeted funding and enhanced logistical support to ensure equitable access, particularly in rural and insecure areas. This study provides evidence to inform future vaccination campaigns and supports decision-making for typhoid conjugate vaccine introduction in other countries in the region.

PLOS ONE

Multi-objective gold rush optimization algorithm: Theoretical Extensions and applications in UAV path planning

by Kecheng Su, Yaoyang Wang, Yikang Kong, Wenan Liu Multi-objective optimization problems have extensive application value in the fields of engineering and science, among which UAV path planning, as a typical application scenario, has attracted considerable attention. This study innovatively proposes a multi-objective extension of the Gold Rush Optimization algorithm (GRO), namely the Multi-Objective Gold Rush Optimization algorithm (MOGRO). By introducing a reference-point-guided two-level selection mechanism and an external archive strategy, the algorithm effectively addresses the challenge of obtaining Pareto-optimal solutions in multi-objective optimization problems. A systematic validation method is adopted: firstly, a comparative analysis is conducted between MOGRO and seven advanced multi-objective optimization algorithms on a benchmark test set consisting of 18 standard test problems. Subsequently, a UAV path planning multi-objective optimization model is constructed, taking into account both path length and obstacle threats, and the top four algorithms from the benchmark tests are selected for application validation. Experimental results show that the MOGRO algorithm significantly outperforms the comparison algorithms in terms of convergence, distribution, and solution quality, demonstrating excellent optimization performance. This study not only enriches the theoretical system of the GRO algorithm but also provides an innovative solution for UAV path planning in complex environments, with important theoretical value and practical significance.

PLOS ONE

Clinical usefulness of repeated sputum culture for the identification of pneumonia pathogens: A retrospective study

by Ji Hyun Yun, Jiho Park, Hyun Kyun Ki Sputum culture is essential for identifying pneumonia pathogens, but its sensitivity remains limited. This study evaluated the clinical utility of repeated sputum cultures and factors associated with detecting additional significant isolates in subsequent cultures. We retrospectively analyzed adult patients with pneumonia who underwent three serial sputum cultures at a tertiary hospital between January 2021 and December 2022. We excluded patients with over 48 h interval between each sputum sample or those with coronavirus disease 2019. Initial and subsequent culture results were compared, and clinical factors associated with detecting additional significant isolates were evaluated. A total of 132 patients were included. Sputum quality (p = 0.71) and culture positivity rates (p = 0.25) were similar across serial cultures. Fungi, particularly Candida species, were more frequently isolated from subsequent cultures (p = 0.02). The cumulative detection rate of significant isolates did not improve, although the cumulative number was higher in third than initial cultures (p = 0.01). Hypertension, inappropriate empirical antibiotics, shorter intervals to repeat culture, and greater pneumonia severity were independent risk factors for detecting additional significant isolates. Serial sputum cultures provided limited additional diagnostic information and were not associated with meaningful improvement in the sputum quality, culture positivity, or cumulative detection of significant isolates.

PLOS ONE

Real-world outcomes after switching to faricimab in treatment-resistant diabetic macular edema: A 1-year observational study with choroidal thickness assessment

by Ryo Tomemori, Hidetsugu Mori, Mari Tanaka, Tatsunori Kiriishi, Masatoshi Omi, Yuki Hattori, Shimpei Oba, Masayuki Ohnaka, Hisanori Imai Approximately 40% of diabetic macular edema (DME) cases are resistant to conventional anti-vascular endothelial growth factor (VEGF) therapy. Although faricimab (an antibody against VEGF-A and angiopoietin-2 [Ang-2]) has shown efficacy in treatment-naïve DME, real-world evidence on switching strategies in resistant cases remains limited. We evaluated the 1‑year real‑world effectiveness of switching to faricimab in resistant DME and explored central choroidal thickness (CCT) changes and their relationship to retinal and choroidal responses. This retrospective study included 28 eyes from 19 patients with treatment-resistant DME who switched from aflibercept or ranibizumab to faricimab between May 2022 and August 2023. Central retinal thickness (CRT), CCT, best-corrected visual acuity (BCVA), and injection frequency were evaluated 1 year before, immediately before, and 1 year post-switching. Mean CRT significantly decreased from pre-switching levels of 453 ± 112 μm to 322 ± 60 μm 1-year post-switching (28.9% reduction). The dry macula rate (CRT ≤ 325 μm) was achieved in 53.6% of eyes at 1 year. Injection frequency was significantly higher in the year after switching than in the year before switching (3.5 vs. 5 injections). LogMAR BCVA showed no significant change (0.31 ± 0.27 vs. 0.34 ± 0.32). Ellipsoid zone (EZ)/external limiting membrane (ELM) disruption increased from 28.6% at 1 year before switching to 35.7% immediately before switching, with minimal progression to 39.3% at 1 year post-switching. Mean CCT demonstrated no significant change (238 ± 82 μm vs. 230 ± 62 μm). Faricimab achieved significant anatomical improvements in resistant DME supporting its efficacy in real-world practice. Stable CCT may indicate preserved choroidal perfusion and represent a favorable characteristic of dual VEGF-A and Ang-2 inhibition. The potential suppression of the outer retinal structural deterioration may represent a marker of vascular health during chronic anti-VEGF therapy.

PLOS ONE

The association between upper limb function, physical exercise, and cognitive ability among empty-nest elderly in China: A cross-sectional study based on CLHLS

by Quan Zhou, Jiaran Jiang, Chuanxia Zhang, Yiming Ma Background The rapid aging of China’s population poses serious challenges to the cognitive health of older adults living without co-resident children (empty-nest older adults). Currently, the role of upper limb function on the cognitive function of this group and its potential pathway of effect through physical exercise remain unclear. Objective This study aimed to explore the associations among upper limb function, physical exercise, and cognitive ability in the Chinese empty-nest elderly population, and to explore the potential mediating role of physical exercise in the relationship between upper limb function and cognition. Due to the cross-sectional design, no causal relationships can be established; all reported associations are statistical in nature. Methods This study employed a cross-sectional design using data from the 2018 Chinese Longitudinal Healthy Longevity Survey (CLHLS). Based on household structure information, older adults aged 60 and above who did not live with their children were defined as “empty-nest elderly”. A total of 5,060 empty-nest elderly were included in the final analysis. Upper limb function (normal/restricted), physical exercise (yes/no), and cognitive ability (normal/impaired) were assessed via questionnaire. Analyses included multivariable logistic regression, restricted cubic spline curves, Bootstrap-based mediation effect analysis, and subgroup analysis. Results Among the 5,060 participants (mean age 78.60 ± 10.31 years), 755 (14.92%) had cognitive impairment. Multivariable-adjusted analysis showed that restricted upper limb function was significantly positively associated with the risk of cognitive impairment (OR=2.55, 95% CI: 1.95–3.29), while regular physical exercise was significantly negatively associated with this risk (OR=0.77, 95% CI: 0.62–0.95). Mediation analysis indicated that physical exercise accounted for 5.95% of the total effect (ACME = 0.0121, 95% CI: 0.0074–0.0172). Given the small effect size, this pathway should be interpreted as a minor statistical contributor rather than a dominant mechanism. Subgroup analysis showed that the association of upper limb function and physical exercise with cognitive impairment was significantly modified by drinking status (both P for interaction < 0.05), with stronger effects in non-drinkers. Conclusion Among Chinese empty-nest elderly, restricted upper limb function is positively associated with the risk of cognitive impairment, while regular physical exercise is negatively associated with this risk. Physical exercise accounts for a minor portion of the statistical association between upper limb function and cognitive ability.

PLOS ONE

Barriers, motivations, and understanding: A qualitative study on the acceptability of an academic weight management program

by Yongjia Deng, Kristen Moore, Patricia Galanti, Laura Davisson, Nasser Alrayyes, Cristhian Perez, Patricia Dekeseredy, Treah Haggerty Introduction Obesity is a critical health issue, and West Virginia is a state with an exceptionally high obesity rate. There are evidence-based medical guidelines for treating obesity utilized by medical weight management programs. The primary purpose of this study is to gain a comprehensive understanding of the acceptability of an academic medical weight management program that utilizes guidelines-based treatment for obesity. Methods This study employs a qualitative descriptive approach. Semi-structured interviews were completed with 20 participants enrolled in an academic medical weight management program. Interviews were completed and transcribed. Themes were identified through qualitative content analysis. Results Twenty participants enrolled in a medical weight management clinic participated. Five main themes emerged during analysis of the interview content: 1) satisfaction with clinical and support staff, 2) desire for additional touchpoints with clinical staff, 3) perception of non-judgmental support, 4) perception of support by the clinic following policy changes affecting treatment coverage, and 5) participation due to intrinsic motivation. Conclusion Patients enrolled in medical weight management clinics exhibit a wide diversity of motivations for their desired weight loss, but commonly face a variety of challenges to their care, including unreliable insurance coverage and social stigma related to obesity. However, the resulting themes identify acceptable approaches for medical weight management clinics such as increase touch points, supportive clinical staff who provide non-judgmental support, and an understanding of participants intrinsic motivations.

PLOS ONE

Surface modification of carbon-based adsorbents derived from coal gasification slag for the efficient adsorption of trimethoprim

by Zhiqiang Yang, Jia Liu, Qingchao Li Given that pristine water bodies are irreplaceable for maintaining ecosystem homeostasis, the remediation of antibiotic-contaminated aquatic systems has emerged as a pivotal challenge. This research used flotation residual carbon (FRC) obtained from coal gasification coarse slag (CGCS) as a precursor and adopted the H2O2 liquid-phase oxidation method to prepare a low-cost and efficient adsorbent by optimizing process parameters such as modification temperature, time, and oxidant concentration. The H2O2 treated flotation residual carbon was denoted as HFRC. Batch adsorption assays revealed that, under their respective optimal conditions, equilibrium uptakes of Trimethoprim (TMP) by FRC and HFRC reached 54.02 mg/g and 80.01 mg/g, with equilibrium times of 270 min and 360 min. Comprehensive characterization, molecular simulations, and model fitting collectively elucidated the adsorption mechanism and the kinetic intensification of TMP. H2O2 modification markedly increased the abundance of oxygen-containing functionalities. On this basis, the van der Waals and electrostatic interactions between HFRC and TMP were significantly enhanced, accompanied by a pronounced increase in hydrogen-bond density, which collectively accelerated the TMP adsorption kinetics. HFRC also exhibited exceptional dynamic adsorption performance in the fixed-bed column. Overall, HFRC integrates rapid removal performance with resource-cycling benefits and superior environmental adaptability, offering an economically and ecologically synergistic paradigm for the valorization of CGCS and the remediation of antibiotic-impaired waters.

PLOS ONE

Associations of CYP2B6, CYP2C19, and CYP2D6 isoforms and phenoconversion with sertraline pharmacokinetics

by Begoña Tapia-Alzuguren, Covadonga Canga-Espina, Patricio Molero, Azucena Aldaz Interindividual variability in sertraline exposure is substantial and may be influenced by genetic variation in drug-metabolizing enzymes as well as by concomitant medications that modify enzyme activity. This study explored the association between genetic variability in the cytochrome P450 isoforms CYP2B6, CYP2C19, and CYP2D6 and sertraline pharmacokinetics in a real-world clinical setting, accounting for medication-related phenotype modification. We conducted an observational case-series analysis including seven hospitalized patients with available pharmacogenetic data and sixteen therapeutic drug monitoring measurements of sertraline. Pharmacokinetic parameters were descriptively compared across genotype-predicted metabolic phenotypes and after adjustment for concomitant medications with potential inhibitory or inducing effects, as informed by available interaction data. Before accounting for medication-related phenotype modification within a descriptive framework, variability in sertraline exposure appeared to follow patterns consistent with genotype-predicted phenotypes related to CYP2B6, whereas limited or inconsistent patterns were observed for CYP2C19 and CYP2D6. After incorporating co-medication effects, patterns across sertraline serum concentrations, concentration-to-dose ratios, and apparent clearance were more consistent for adjusted CYP2B6 phenotypes. Adjusted CYP2C19 phenotypes showed additional variability patterns in dose-normalized exposure and clearance, while no consistent associations were observed for CYP2D6 after adjustment. The metabolite-to-parent compound ratio showed no clear relationship with either genetic or adjusted phenotypes. These findings suggest that interpretation of pharmacogenetic information for sertraline may benefit from integrating CYP2B6 and CYP2C19 genotype data with concomitant medication use. In this exploratory case series, accounting for medication-related phenotype modification revealed variability patterns that were less evident when genetic information was considered alone. Current guidelines consider CYP2B6 and CYP2C19 actionable for sertraline; our findings suggest that integrating co-medication–informed interpretation with pharmacogenetic testing and therapeutic drug monitoring may further refine individualized assessment of sertraline exposure in complex clinical settings.

PLOS ONE

Gaelic games coaches’ attitudes towards, awareness of and use of injury prevention exercise programmes

by Calvin Teahan, Enda Whyte, Siobhán O’Connor Purpose Gaelic games (GG) are multidirectional, evasive sports with unpredictable high-intensity activity. Lower extremity injuries are common. Injury prevention exercise programmes (IPEPs) have been introduced, effectively reducing injuries and increasing neuromuscular performance. However, the awareness and use of IPEPs are anecdotally low among coaches. Methods Adult GG coaches (n = 342) completed an anonymous online survey, including awareness, use, and attitudes towards IPEPs and injury prevention. Frequencies and descriptive statistics were conducted, a chi-squared test was used to assess any differences in awareness and use of IPEPs, a Mann-Whitney U test was used to examine differences between groups for attitudes to injury prevention. Results Overall, 59.5% (n = 165) of coaches reported using any form of an IPEPs, including modified or self-developed programmes, while 44.1% of coaches (n = 130) were aware of formal IPEPs. Use was statistically higher in elite coaches than non-elite (p < 0.01, phi = 0.16). No significant differences in IPEP use were observed by coach gender, team gender, or coaching education. Many coaches developed their own IPEP or altered a current IPEP, particularly participants coaching females. Coaches had a positive attitude towards injury prevention, but 40.6% stated a lack of knowledge on how to use an IPEP, with 34.5% agreeing there was no training available to teach them. Discussion Despite generally positive attitudes towards injury prevention, awareness and implementation of formal IPEPs among GG coaches remain limited. These findings highlight the need for targeted coach education and improved dissemination strategies to enhance the adoption and effective implementation of IPEPs in GG, which is critical to mitigating the risk of injury.

PLOS ONE

Characteristics of long stay home care clients’ acute care use who live with frailty in Alberta, Canada: A retrospective cohort study

by Jananee Rasiah, Andrea Gruneir, Jeffrey Poss, Jayna Holroyd-Leduc, Greta G. Cummings Objective To describe the characteristics of long stay home care clients’ acute care use who live with frailty, and to compare associated factors for hospitalizations. Methods The cohort comprised individuals 65 to 104 years old (n=10,107) who were observed from 2015-2017. Population-based administrative data were linked by individual records, descriptive statistics were reported, and logistic regression models were run. Results Majority of clients were female (69.7%), and the largest proportion of clients were 75-84 years old. Arthritis (65.6%) and hypertension (74.5%) were most prevalent in clients who were frail. Frail clients (40.6%) had between 1-3 emergency department visits. Mean length of stay was 16.6 days for frail hospitalized clients. Being pre-frail and frail were associated factors for hospitalization. Conclusion Further research is needed to determine the risk classification of more factors, such as social characteristics that predispose home care clients with frailty to hospitalization.

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