Directional bias of a single polarized cell under confinement

Chiral patterns have been observed in various processes from swirling bacterial colonies to tissue morphogenesis and cytoskeletal organization, yet the physical mechanisms underlying chiral cell motion remain poorly understood.

Motivated by experiments demonstrating directional bias in the circular motion of confined cells, we use the tools of dynamical systems analysis with computer simulations to identify minimal intrinsic and extrinsic mechanisms capable of generating persistent biased migration.

The dynamical systems framework reveals a common organizing principle: directional bias emerges through changes in the stability and/or basins of attraction of the clockwise and counter-clockwise motility states.

We find four distinct routes to such bias.

First, intrinsic torque in a polarized cytoskeleton can be spatially integrated to produce biased circular motion.

Second, anisotropic cell-substrate friction can generate directional preference when reduced friction along the polarity axis is coupled to a directional offset.

Third, a chiral wall-alignment response can also produce a persistent directional preference.

Finally, substrate patterns that break mirror symmetry, such as dextral or sinistral ridges and troughs, can likewise bias rotational direction.

Together, these mechanisms yield distinct, testable predictions and suggest a unifying lens for experimental interrogation of cellular chirality and the design of synthetic systems with programmable chiral motion.