Multifractal Scaling in Hi-C Maps

The three-dimensional organization of the genome exhibits rich, scale-dependent structure, as revealed by both chromosome contact maps (e.g., Hi-C maps) and chromatin density measured by microscopy.

Recent studies have reported multifractal scaling in these data.

Yet, the origin of this scaling behavior remains unclear: existing efforts describe it through postulated models.

Here, we show that the multifractal structure of Hi-C maps is a direct consequence of the power-law contact probability $P(s)$, which is itself an empirical observable measured from Hi-C maps.

Starting from $P(s)$ with a single exponent $\gamma$, we analytically derive the mass exponent $\tau(q)$, which characterizes how the $q$-th moment of contact density scales with box size $l$ used to coarse-grain the genomic coordinate.

This multifractal behavior reflects the geometric competition between intra- and inter-segment contacts.

We find that the slope of $\tau(q)$ at large $q$ is given by $2 -\gamma$ when $\gamma <1$, and by $1$ when $\gamma \geq 1$.

We further show that this behavior is robust to noise and consistent across diverse organisms, indicating that it is a universal feature of chromatin organization.

We extend our analysis into double-exponent $P(s)$, and show the $l$ dependence in multifractal behavior.

Taken together, these results provide a physical explanation for multifractal scaling and establish a direct link between the multifractality in Hi-C maps and polymer contact statistics, with the large-$q$ slope of $\tau(q)$ mapping onto a known polymer contact exponent.