Plasmon-Enabled High-Precision Single Molecule Localization Microscopy over an Extended Field of View

We propose PIFLUX, a single-molecule localization scheme combining deep-subwavelength plasmonic illumination with widefield detection.

Interference between counter-propagating gap plasmons and a normally incident optical field generates an illumination pattern whose position can be tuned through the plasmon phase while preserving its spatial period.

A Cramér-Rao analysis shows PIFLUX reaches few-nanometer precision matching MINFLUX while doubling that of SIMFLUX over a micrometer field of view, and a maximum-likelihood estimator confirms this on a synthetic nuclear pore complex.